Greenlight Biosciences


Zepteon has developed Glycap-3A, a unique, breakthrough, patent pending (WO2013013193 and US 20130084648 A1) affinity purification technology that enables separation of antibodies based on their fucose levels. The affinity purification resin contains optimally immobilized FcgammaR3A receptors, leading to high antibody equilibrium binding capacities. The percentages of nonfucosylated antibodies have been highly enriched using these resins, as shown below

Bolton et. al, Biotech Prog, April 2013; btpr.1717

Pace et. al, Biotech Prog, June 2016; btpr.2300


Hyperimmune IgG is typically obtained from human donors who have been exposed to a virus and have generated virus specific antibodies. Literature indicates hyperimmune IgG would be far more potent against a range of viruses (Flu, MERS-CoV, Ebola, HPV, West Nile, CHIKV) using optimal IgG glycoforms and subclasses (Zeitlin et. al, PNAS, 2011; doi: 10.1073/pnas.1108360108).

Anti-viral plasma IgG fractionated using the Glycap-3A resin has been demonstrated to be far more potent against viral infection in mice. Zepteon resin is the only technology capable of simultaneously providing more potent IgG glycoforms and subclasses (IgG1 and IgG3) for the $1B USD hyperimmune IgG market. The resin can be used to rapidly obtain therapies after a viral outbreak. The method is far faster than the generation, testing and scale up of monoclonal antibody therapies.

The problem biosimilar monocloncal antibody makers have to solve is matching the properties of the original drug. During manufacturing, antibodies are produced in cells that attach various sugar structures like fucose. The FDA and EMA have indicated that biosimilar antibody manufacturers should compare receptor binding of their candidate to that of the innovator drug. Biosimilar drugs with equal nonfucosylation levels as those of the innovator drugs would have similar receptor binding and therefore be likely to gain regulatory approval. The Zepteon Glycap-3A, affinity purification technology that addresses this critical need for biosimilars makers by enabling separation of antibodies based on their fucose levels.

Zepteonís Glycap-3A resin can be used to make a biosimilar monocloncal antibody with a nonfucose level that matches that of the original drug, or one with a nonfucose level at the upper end of the range of the original drug for use in structure function studies (Pace et. al, Biotech Prog, June 2016; btpr.2300). The economical, fast and robust Zepteon separation method is superior to cellular methods of targeting fucose levels. Cellular methods like genetically engineering cells or screening cell lines and bioreactor conditions are slow, complex and costly. The glycoforms obtained using the Zepteon resin are the most physiologically relevant since the separation is performed using natural human receptors.